AbstractText: Zika virus (ZIKV) infections have been linked to different levels of clinical outcomes ... response, focusing on the immune mediators profile in 95 acute ZIKV-infected adult patients from Campinas, Brazil... hepatocyte growth factor than those without such abnormalities born to ZIKV-infected mothers AbstractText: This study identified several key markers for the control of ZIKV pathogenesis ... will allow a better understanding of the molecular mechanisms of ZIKV infection in patients Keyword: Zika virus.
Infections (3), Nervous System Malformations (2), Fetal Growth Retardation (1), more mentions
Abstract: Zika virus (ZIKV), a mosquito-borne flavivirus, causes devastating congenital birth defects ... monoclonal antibody (mAb), ZKA190, that potently cross-neutralizes multi-lineage ZIKV strains... highly effective in vivo in preventing morbidity and mortality of ZIKV-infected mice... 1 robustly prevented viral escape, warranting its development as a ZIKV immunotherapy Keyword: Zika.
DescriptorName: Zika Virus. DescriptorName: Zika Virus Infection... the neurodevelopmental abnormalities observed in neonates exposed in utero to Zika virus (ZIKV) is needed to develop treatments... OCT) can provide an insight into the pathophysiology of congenital Zika syndrome (CZS) AbstractText: To quantify the microstructural changes of the ... degeneration was similar to that of cblC deficiency AbstractText: Congenital Zika syndrome showed a central retinal degeneration with severe GCL loss ...
Retinal Degeneration (3), Ganglion Cysts (2), Infections (2), more mentions
... Croix, had reported 1,021 probable or confirmed cases* of Zika virus disease in its population of approximately 100,000 (1 ... asymptomatic pregnant women in the USVI had tested positive for Zika virus... Interventions included education and outreach, distribution of Zika prevention kits(†) to pregnant women in the USVI, and provision of free Zika virus laboratory testing and vector control services ...
Abstract: Zika virus (ZIKV) is a major public health concern due to its overwhelming ... licensed vaccines nor antiviral therapies available for the treatment of ZIKV... aimed to identify and rationally optimize effective therapeutic regimens for ZIKV by evaluating the antiviral potential of approved broad-spectrum antiviral ... For these studies, Vero cells were infected with ZIKV in the presence of increasing concentrations of FAV, IFN, or ...
Abstract: The Zika virus crisis exemplified the risk associated with emerging pathogens and ... we review all data reported during the unexpected emergence of Zika virus in French Polynesia in late 2013... retrospective description of CNS malformations in neonates, the isolation of Zika virus in semen, the potential for blood-transfusion transmission, mother ...
Abstract: Background Although Zika virus (ZIKV) infection is typically self-limiting, other associated complications such as ... There are no approved vaccines against ZIKV infection... of a synthetic, consensus DNA vaccine (GLS-5700) encoding the ZIKV premembrane and envelope proteins in two groups of 20 participants ... On neuronal-cell assay, there was 90% inhibition of ZIKV infection in 70% of the serum samples and 50% inhibition ...
Vaccines (9) Infections (3), Congenital Abnormalities (1), more mentions
... disease manifestations including microcephaly, congenital malformation, and Guillain-Barré syndrome, Zika virus (ZIKV) remains a persistent global public health threat... extended CD-loop and hydrogen-bonding interaction network within the ZIKV envelope protein contribute to stability differences between the viral families... infection, disease manifestation, and persistence in body fluids seen following ZIKV infection.
Vaccines (2) Infections (3), Dengue (1), Microcephaly (1), more mentions
Abstract: The rise of Zika virus (ZIKV) and its unusual clinical manifestations provided ground for speculative debate ... was recently suggested that previous exposure to DENV may worsen ZIKV clinical outcomes... cohorts demonstrating that there is no correlation to ADE when ZIKV infection occurs in the presence of pre-existing DENV immunity.
Abstract: Zika virus (ZIKV) is an arthropod-borne virus mainly transmitted by Aedes species ... A total of nine of the 16 imported ZIKV reported cases during the mosquito season in the Republic of ... Korea (ROK), following the return of local nationals from foreign ZIKV endemic countries, were surveyed for Aedes albopictus.
The new viruses, designated La Tina, Kampung Karu, and Long Pine Key, respectively, are antigenically and phylogenetically more similar to the mosquito-borne flavivirus pathogens, than to the classical insect-specific viruses like cell fusing agent and Culex flavivirus. The potential implications of this relationship and the possible uses of these and other arbovirus-related insect-specific flaviviruses are reviewed.
Background: We assessed replication and excretion of the live attenuated tetravalent dengue vaccine (CYD-TDV) into biological fluids following vaccination in dengue-naive adults in Australia.
Methods: Vaccinal viremia/shedding was assessed in a subset of participants enrolled in a lot-to-lot consistency study; 95 participants received 3 subcutaneous doses of CYD-TDV from phase 2/3 lots of the vaccine, and 8 received placebo; doses were administered 6 months apart. Quantitative reverse-transcription polymerase chain reaction (qR-PCR) analysis was used to initially detect the yellow fever virus (YFV) core protein gene in the backbone of CYD-TDV in serum, saliva and urine, followed by serotype-specific qRT-PCR analysis of samples positive for YFV by qRT-PCR (lower limit of detection, 5.16 GEq/mL).
Results: YFV viremia was detected by qRT-PCR in 69.5% of participants (66 of 95) who received CYD-TDV, mainly 6-14 days after injection 1. The serotypes detected were serotype 4 (in 68.2% of participants [45 of 95]), serotype 3 (in 19.7% [13 of 95]), and serotype 1 (in 12.1% [8 of 95]); serotype 2 was not detected. None of the placebo recipients had vaccinal viremia/shedding. No participants had detectable viral shedding into saliva at levels above the lower limit of quantitation. Two participants had low-level viral shedding (serotype 3) in urine (5.47 and 5.77 GEq/mL). None of the participants with viremia or shedding experienced concomitant fever.
Conclusions: Low-level vaccinal viremia may occur following vaccination with CYD-TDV, but this is not associated with any symptom or adverse event.
Clinical Trials Registration: NCT01134263.
However, little is known about the detailed mechanisms of viral replication and pathogenicity in the brain. Previously, we reported that the genomic RNA of tick-borne encephalitis virus (TBEV), a member of the genus Flavivirus, is transported and replicated in the dendrites of neurons... Keyword: flavivirus. Keyword: neuronal granule. Keyword: neuropathogenicity.
... within ex vivo I. scapularis organs and provide a significant translational tool for evaluating recombinant, live vaccine candidates and potential tick transcripts and proteins for possible therapeutic use and vaccine development to reduce TBFV transmission.IMPORTANCE Tick-borne flavivirus (TBFV) infections cause neurological and/or hemorrhagic disease in humans worldwide.
Genetic epilepsies are caused by mutations in a range of different genes, many of them encoding ion channels, receptors or transporters. While the number of detected variants and genes increased dramatically in the recent years, pleiotropic effects have also been recognized, revealing that clinical syndromes with various degrees of severity arise from a single gene, a single mutation, or from different mutations showing similar functional defects. Accordingly, several genes coding for GABAA receptor subunits have been linked to a spectrum of benign to severe epileptic disorders and it was shown that a loss of function presents the major correlated pathomechanism. Here, we identified six variants in GABRA3 encoding the α3-subunit of the GABAA receptor. This gene is located on chromosome Xq28 and has not been previously associated with human disease. Five missense variants and one microduplication were detected in four families and two sporadic cases presenting with a range of epileptic seizure types, a varying degree of intellectual disability and developmental delay, sometimes with dysmorphic features or nystagmus. The variants co-segregated mostly but not completely with the phenotype in the families, indicating in some cases incomplete penetrance, involvement of other genes, or presence of phenocopies. Overall, males were more severely affected and there were three asymptomatic female mutation carriers compared to only one male without a clinical phenotype. X-chromosome inactivation studies could not explain the phenotypic variability in females. Three detected missense variants are localized in the extracellular GABA-binding NH2-terminus, one in the M2-M3 linker and one in the M4 transmembrane segment of the α3-subunit. Functional studies in Xenopus laevis oocytes revealed a variable but significant reduction of GABA-evoked anion currents for all mutants compared to wild-type receptors. The degree of current reduction correlated partially with the phenotype. The microduplication disrupted GABRA3 expression in fibroblasts of the affected patient. In summary, our results reveal that rare loss-of-function variants in GABRA3 increase the risk for a varying combination of epilepsy, intellectual disability/developmental delay and dysmorphic features, presenting in some pedigrees with an X-linked inheritance pattern.
Neurological and Central Nervous System Diseases (4), Neuroscience (1) Epilepsy (5), Intellectual Disability (4), Facies (1), more mentions